Through the analysis of the budding rate and cell growth line graphs, we found that knocking out the 193C gene promotes cell division in the early stages and maintains high cell viability. However, in the later stages, it somewhat slows down cellular activity, resulting in a decrease in the budding rate. The knockout of RPL36B and its IncRNA YPL250W-A can extend cell viability and maintain a higher budding rate, thereby delaying the aging process of cells. At the same time, I observed that knocking out the YPL250W-A gene initially reduces cell viability but increases it in later stages; additionally, the budding rate also rises in later stages. I infer that knocking out 250W delays cellular aging.
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