Through the analysis of the cell cycle proportion diagram, it was found that the knockout of 193C overall promotes cell division and delays cellular aging. At the same time, for the knockout of RPL36B and its related IncRNA YPL250W-A, it was observed that in the early to mid-phase, there is promotion of cell division; however, in the later phase, the number of cells entering mitosis decreases dramatically, which may be associated with gene knockout. Specifically, knocking out the YPL250W-A gene promotes cell division in the early stage and shows a decrease in mid-stage; although there is an overall decline in division capability in the later stage, knockout of YPL250W-A still promotes cell division and delays the aging process.
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