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Practical 1: RT-PCR / qRT-PCR Analysis of ZEB1 in Breast Cancer Cells PRACTICAL SUMMARY OVERVIEW In this practical, we will carry out RT-PCR and qRT-PCR analysis of human breast cancer cell samples. Expression of ZEB1, a gene implicated in cancer ...

Based on the information provided, it is difficult for me to formulate a specific hypothesis without additional details or knowledge of the experimental setup. However, I can provide some general guidance on how you could approach formulating a hypothesis for this experiment.

To devise a hypothesis, you should consider the known functions and roles of ZEB1 in breast cancer progression. For example, if previous studies have shown that higher expression levels of ZEB1 are associated with increased metastatic potential in breast cancer cells, your hypothesis could be that MDA-MB-231 cells (more aggressive) will exhibit higher ZEB1 expression compared to MCF-7 cells (less aggressive).

Once you have formulated your hypothesis, you can evaluate whether the results from RT-PCR and qRT-PCR analysis support your hypothesis. Analyze the gel image and check if there is a clear difference in band intensity for ZEB1 between the two cell lines. Similarly, examine the qRT-PCR data table to see if there is a higher relative expression level of ZEB1 in MDA-MB-231 compared to MCF-7.

If both the gel image and qRT-PCR data show consistent results with your hypothesis (i.e., higher ZEB1 expression in more aggressive cells), then it would suggest that your hypothesis is supported by the experimental findings. However, it’s important to conduct statistical analysis and replicate the experiment multiple times to ensure reliable conclusions.

Remember that formulating hypotheses and interpreting experimental results require careful consideration of prior knowledge, experimental design, and analysis methods.


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